protease 1 senp1 catalytic domain (R&D Systems)
Structured Review

Protease 1 Senp1 Catalytic Domain, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protease+1+senp1+catalytic+domain/Recombinant+Human+His6-SENP1+Catalytic+Domain+Protein%2C+CF/pmc11335207-182-36-41
Average 93 stars, based on 10 article reviews
Images
1) Product Images from "Protein SUMOylation promotes cAMP-independent EPAC1 activation"
Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation
Journal: Cellular and Molecular Life Sciences: CMLS
doi: 10.1007/s00018-024-05315-y
Figure Legend Snippet: Heat shock promotes EPAC1 post-translational modifications (PTM). Levels of cellular EPAC1 PTM probed by immunoblotting using anti-EPAC1 antibody in HUVEC ( A ) and HEK293/EPAC1-Flag B in response to heat shock as a function of time. C Levels of cellular EPAC1 PTM in HEK293/EPAC1-EYFP cells probed by immunoblotting using anti-EPAC1 antibody, with and without heat shock (30 min) and with or without SENP1 (220 nM) treatment at 37 °C for 20 min. Similar results were obtained from at least three independent experiments
Techniques Used: Western Blot
Related Articles
Lysis:Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1 × cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation. Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1 × cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1× cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific Incubation:Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1 × cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation. Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1 × cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1× cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific Recombinant:Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1 × cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation. Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1 × cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1× cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific Western Blot:Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1 × cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation. Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1 × cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1× cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific |