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protease 1 senp1 catalytic domain  (R&D Systems)


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    Structured Review

    R&D Systems protease 1 senp1 catalytic domain
    Heat shock promotes EPAC1 post-translational modifications (PTM). Levels of cellular EPAC1 PTM probed by immunoblotting using anti-EPAC1 antibody in HUVEC ( A ) and HEK293/EPAC1-Flag B in response to heat shock as a function of time. C Levels of cellular EPAC1 PTM in HEK293/EPAC1-EYFP cells probed by immunoblotting using anti-EPAC1 antibody, with and without heat shock (30 min) and with or without <t>SENP1</t> (220 nM) treatment at 37 °C for 20 min. Similar results were obtained from at least three independent experiments
    Protease 1 Senp1 Catalytic Domain, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protease+1+senp1+catalytic+domain/Recombinant+Human+His6-SENP1+Catalytic+Domain+Protein%2C+CF/pmc11335207-182-36-41
    Average 93 stars, based on 10 article reviews
    protease 1 senp1 catalytic domain - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Protein SUMOylation promotes cAMP-independent EPAC1 activation"

    Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-024-05315-y

    Heat shock promotes EPAC1 post-translational modifications (PTM). Levels of cellular EPAC1 PTM probed by immunoblotting using anti-EPAC1 antibody in HUVEC ( A ) and HEK293/EPAC1-Flag B in response to heat shock as a function of time. C Levels of cellular EPAC1 PTM in HEK293/EPAC1-EYFP cells probed by immunoblotting using anti-EPAC1 antibody, with and without heat shock (30 min) and with or without SENP1 (220 nM) treatment at 37 °C for 20 min. Similar results were obtained from at least three independent experiments
    Figure Legend Snippet: Heat shock promotes EPAC1 post-translational modifications (PTM). Levels of cellular EPAC1 PTM probed by immunoblotting using anti-EPAC1 antibody in HUVEC ( A ) and HEK293/EPAC1-Flag B in response to heat shock as a function of time. C Levels of cellular EPAC1 PTM in HEK293/EPAC1-EYFP cells probed by immunoblotting using anti-EPAC1 antibody, with and without heat shock (30 min) and with or without SENP1 (220 nM) treatment at 37 °C for 20 min. Similar results were obtained from at least three independent experiments

    Techniques Used: Western Blot

    Related Articles

    Lysis:

    Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation
    Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1 × cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis. ..

    Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation.
    Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1 × cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis. ..

    Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation
    Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1× cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis. ..

    Incubation:

    Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation
    Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1 × cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis. ..

    Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation.
    Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1 × cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis. ..

    Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation
    Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1× cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis. ..

    Recombinant:

    Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation
    Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1 × cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis. ..

    Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation.
    Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1 × cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis. ..

    Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation
    Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1× cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis. ..

    Western Blot:

    Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation
    Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1 × cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis. ..

    Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation.
    Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1 × cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis. ..

    Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation
    Article Snippet: .. To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1× cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis. ..



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    Image Search Results


    Heat shock promotes EPAC1 post-translational modifications (PTM). Levels of cellular EPAC1 PTM probed by immunoblotting using anti-EPAC1 antibody in HUVEC ( A ) and HEK293/EPAC1-Flag B in response to heat shock as a function of time. C Levels of cellular EPAC1 PTM in HEK293/EPAC1-EYFP cells probed by immunoblotting using anti-EPAC1 antibody, with and without heat shock (30 min) and with or without SENP1 (220 nM) treatment at 37 °C for 20 min. Similar results were obtained from at least three independent experiments

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Protein SUMOylation promotes cAMP-independent EPAC1 activation

    doi: 10.1007/s00018-024-05315-y

    Figure Lengend Snippet: Heat shock promotes EPAC1 post-translational modifications (PTM). Levels of cellular EPAC1 PTM probed by immunoblotting using anti-EPAC1 antibody in HUVEC ( A ) and HEK293/EPAC1-Flag B in response to heat shock as a function of time. C Levels of cellular EPAC1 PTM in HEK293/EPAC1-EYFP cells probed by immunoblotting using anti-EPAC1 antibody, with and without heat shock (30 min) and with or without SENP1 (220 nM) treatment at 37 °C for 20 min. Similar results were obtained from at least three independent experiments

    Article Snippet: To test if EPAC1 PTM was sensitive to SUMO-specific deconjugating enzyme, heat shock treated cells were lysed with 1 × cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis.

    Techniques: Western Blot